EGLN1 antibody

Catalog No. ABIN2461001

The Rabbit Polyclonal anti-EGLN1 antibody has been validated for WB and ELISA. It is suitable to detect EGLN1 in samples from Human, Mouse, Rat and Dog.

Quick Overview for EGLN1 antibody (ABIN2461001)

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGLN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EGLN1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EGLN1.

Application Details

Application Notes: EGLN1 antibody can be used for detection of EGLN1 by ELISA at 1:12500. EGLN1 antibody can be used for detection of EGLN1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store EGLN1 antibody at -20 °C.

Target Details for EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Alternative Name: EGLN1

Background: EGLN1 has a role in the regulation of hypoxia-inducible factor. It is not affected by overexpression or downregulation of HIF-2alpha.

Molecular Weight: 46 kDa

Gene ID: 54583

NCBI Accession: NP_071334

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, Warburg Effect