The Rabbit Polyclonal anti-FUBP1 antibody has been validated for WB and ELISA. It is suitable to detect FUBP1 in samples from Human, Mouse, Rat and Dog.
FUBP1 antibody can be used for detection of FUBP1 by ELISA at 1:62500. FUBP1 antibody can be used for detection of FUBP1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store FUBP1 antibody at -20 °C.
Target
FUBP1
(Far Upstream Element (FUSE) Binding Protein 1 (FUBP1))
Alternative Name
FUBP1
Background
Far upstream element-binding protein activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. FUBP activity may be regulated by alternative splicing, translation efficiency, and posttranslational modification.