Far Upstream Element (FUSE) Binding Protein 1 (FUBP1) (AA 540-589) antibody

Details for Product No. ABIN6741561
Antigen
Epitope
AA 540-589
10
9
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Monkey, Pig (Porcine), Rabbit, Rat (Rattus)
76
38
31
7
6
5
5
4
3
2
2
2
2
1
Host
Rabbit
59
11
7
Clonality
Polyclonal
Conjugate
Un-conjugated
6
4
3
3
3
3
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
64
39
16
9
6
5
5
4
2
2
1
1
Options
Brand IHC-plus™
Immunogen Synthetic peptide located between aa540-589 of human FUBP1 (Q96AE4, NP_003893). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Marmoset, Rat, Elephant, Bovine, Rabbit, Pig (100%), Gorilla, Dog (93%), Mouse, Guinea pig, Stickleback (92%), Galago (87%), Turkey, Chicken, Platypus (85%), Bat (81%).

Type of Immunogen: Synthetic peptide
Isotype IgG
Specificity Human FUBP1
Predicted Reactivity Percent identity by BLAST analysis: Human, Rat, Bovine, Rabbit (100%) Dog (93%) Mouse, Guinea pig (92%) Chicken (85%).
Purification Immunoaffinity purified
Antigen
Alternative Name FUBP1 (FUBP1 Antibody Abstract)
Background Name/Gene ID: FUBP1

Synonyms: FUBP1, DNA helicase V, FBP, HDH V, FUSE-binding protein 1, FUBP
Gene ID 8880
NCBI Accession NP_003893
UniProt Q96AE4
Application Notes Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only
Format Lyophilized
Reconstitution Distilled water
Concentration Lot specific
Buffer Lyophilized from PBS with 2 % sucrose
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Far Upstream Element (FUSE) Binding Protein 1 (FUBP1) (AA 540-589) antibody (ABIN6741561) Human Breast (formalin-fixed, paraffin-embedded) stained with FUBP1 antibody ABIN4621...
Image no. 2 for anti-Far Upstream Element (FUSE) Binding Protein 1 (FUBP1) (AA 540-589) antibody (ABIN6741561) Human Colon (formalin-fixed, paraffin-embedded) stained with FUBP1 antibody ABIN46219...