HMGB3 antibody

Catalog No. ABIN2461205

The Rabbit Polyclonal anti-HMGB3 antibody has been validated for WB and ELISA. It is suitable to detect HMGB3 in samples from Human and Dog.

Quick Overview for HMGB3 antibody (ABIN2461205)

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HMGB3 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HMGB3.

Application Details

Application Notes: HMGB3 antibody can be used for detection of HMGB3 by ELISA at 1:12500. HMGB3 antibody can be used for detection of HMGB3 by western blot at 0.3 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store HMGB3 antibody at -20 °C.

Target Details for HMGB3

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Alternative Name: HMGB3

Background: The human HMG2a gene is transcribed mainly in the placenta. HMG2a encodes a protein which is part of the high mobility group (HMG) family. Members of this family are ubiquitously expressed and facilitate the formation of nucleoprotein complexes where the DNA is sharply bent.

Molecular Weight: 23 kDa

Gene ID: 3149

NCBI Accession: NP_005333

UniProt: O15347