HMGB3 antibody (Internal Region)

Catalog No. ABIN6262302

Product Details anti-HMGB3 Antibody

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGB3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: HMGB3 Antibody detects endogenous levels of total HMGB3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human HMGB3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for HMGB3

Target: HMGB3 (High Mobility Group Box 3 (HMGB3))

Alternative Name: HMGB3 (HMGB3 Products)

Synonyms: HMG-2a antibody, HMG-4 antibody, HMG2A antibody, HMG4 antibody, Hmg2a antibody, Hmg4 antibody, RGD1564407 antibody, hmgb3 antibody, MGC54022 antibody, Xhmgb3 antibody, MGC88931 antibody, fa19b06 antibody, fj43d02 antibody, wu:fa19b06 antibody, wu:fj43d02 antibody, zgc:112073 antibody, HMGB3 antibody, NFD03 antibody, NFD3 antibody, NUCLEOSOME/CHROMATIN ASSEMBLY FACTOR antibody, high mobility group B3 antibody, HMG-1 antibody, HMG2a antibody, HMGB1 antibody, high mobility group box 3 antibody, high mobility group box 3b antibody, high mobility group box 3 S homeolog antibody, high mobility group protein antibody, high mobility group B3 antibody, High mobility group protein B3 antibody, HMGB3 antibody, Hmgb3 antibody, hmgb3 antibody, hmgb3b antibody, hmgb3.S antibody

Background:

Description: Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters (By similarity). Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor (By similarity). Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling (By similarity).

Gene: HMGB3

Molecular Weight: 23 kDa

Gene ID: 3149

UniProt: O15347