DDX50 antibody

Catalog No. ABIN2461361

The Rabbit Polyclonal anti-DDX50 antibody has been validated for WB and ELISA. It is suitable to detect DDX50 in samples from Human and Dog.

Quick Overview for DDX50 antibody (ABIN2461361)

Target: DDX50 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 50 (DDX50))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX50 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DDX50 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDX50.

Application Details

Application Notes: DDX50 antibody can be used for detection of DDX50 by ELISA at 1:62500. DDX50 antibody can be used for detection of DDX50 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store DDX50 antibody at -20 °C.

Target Details for DDX50

Target: DDX50 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 50 (DDX50))

Alternative Name: DDX50

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX50 is a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. DDX50 and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. DDX50 gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined.

Molecular Weight: 81 kDa

Gene ID: 79009

NCBI Accession: NP_076950

UniProt: Q9BQ39