DDX50 antibody (N-Term)

Catalog No. ABIN2775257

This Rabbit Polyclonal antibody specifically detects DDX50 in WB. It exhibits reactivity toward Human, Dog, Cow, Guinea Pig, Horse, Pig and Rabbit.It has been mentioned in 1 publication

Quick Overview for DDX50 antibody (N-Term) (ABIN2775257)

Target: DDX50 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 50 (DDX50))

Reactivity: Human, Dog, Cow, Guinea Pig, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX50 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DDX50 Antibody

Binding Specificity: N-Term

Purpose: DDX50 Antibody - N-terminal region

Sequence: EESESQKKER QKSDRRKSRH HYDSDEKSET RENGVTDDLD APKAKKSKMK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Pig: 93%, Rabbit: 100%

Characteristics: This is a rabbit polyclonal antibody against DDX50. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DDX50

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8098632

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DDX50 antibody (ABIN2775257)

Valdez, Yang, Hong, Sequitin: "Genomic structure of newly identified paralogue of RNA helicase II/Gu: detection of pseudogenes and multiple alternatively spliced mRNAs." in: Gene, Vol. 284, Issue 1-2, pp. 53-61, (2002) (PubMed).

Target Details for DDX50

Target: DDX50 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 50 (DDX50))

Alternative Name: DDX50

Background:

Background Information: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX50 is a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. DDX50 and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. DDX50 gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined.

Gene Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 50

Alternative Symbols: GU2, GUB, mcdrh, RH-II/GuB

Protein Name: ATP-dependent RNA helicase DDX50

Molecular Weight: 81kDa

Gene ID: 79009

NCBI Accession: NP_076950

UniProt: Q9BQ39