DDX54 antibody

Catalog No. ABIN2461362

The Rabbit Polyclonal anti-DDX54 antibody (ABIN2461362) specifically detects DDX54 in WB and ELISA. The antibody is reactive with Human and Dog samples.

Quick Overview for DDX54 antibody (ABIN2461362)

Target: DDX54 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX54 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DDX54 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDX54.

Application Details

Application Notes: DDX54 antibody can be used for detection of DDX54 by ELISA at 1:312500. DDX54 antibody can be used for detection of DDX54 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store DDX54 antibody at -20 °C.

Target Details for DDX54

Target: DDX54 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 54 (DDX54))

Alternative Name: DDX54

Background: DDX54 is a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein interacts in a hormone-dependent manner with nuclear receptors and represses their transcriptional activity.This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene interacts in a hormone-dependent manner with nuclear receptors and represses their transcriptional activity.

Molecular Weight: 97 kDa

Gene ID: 79039

NCBI Accession: NP_076977

UniProt: Q8TDD1

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway