The Rabbit Polyclonal anti-Annexin A2 antibody has been validated for WB and ELISA. It is suitable to detect Annexin A2 in samples from Human, Mouse and Dog.
ANXA2 antibody can be used for detection of ANXA2 by ELISA at 1:312500. ANXA2 antibody can be used for detection of ANXA2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store ANXA2 antibody at -20 °C.
Target
Annexin A2 (ANXA2)
Alternative Name
ANXA2
Background
ANXA2 encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. ANXA2 has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for ANXA2.