E2F3 antibody

Catalog No. ABIN2461642

This Rabbit Polyclonal antibody specifically detects E2F3 in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for E2F3 antibody (ABIN2461642)

Target: E2F3

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-E2F3 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F3.

Application Details

Application Notes: E2F3 antibody can be used for detection of E2F3 by ELISA at 1:62500. E2F3 antibody can be used for detection of E2F3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store E2F3 antibody at -20 °C.

Target Details for E2F3

Target: E2F3

Background: E2F3 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. E2F3 binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.

Molecular Weight: 37 kDa, 49 kDa

Gene ID: 1871

UniProt: Q68DT0