MED27 antibody
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- Target See all MED27 Antibodies
- MED27 (Mediator Complex Subunit 27 (MED27))
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Reactivity
- Human, Mouse, Dog, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MED27 antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB)
- Purification
- Antibody is purified by protein A chromatography method.
- Immunogen
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CRSP8.
- Top Product
- Discover our top product MED27 Primary Antibody
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- Application Notes
- CRSP8 antibody can be used for detection of CRSP8 by ELISA at 1:62500. CRSP8 antibody can be used for detection of CRSP8 by western blot at 5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- Handling Advice
- As with any antibody avoid repeat freeze-thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store CRSP8 antibody at -20 °C.
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- Target
- MED27 (Mediator Complex Subunit 27 (MED27))
- Alternative Name
- CRSP8 (MED27 Products)
- Synonyms
- CRAP34 antibody, CRSP34 antibody, CRSP8 antibody, TRAP37 antibody, CG1245 antibody, Dmel\\CG1245 antibody, Med27 antibody, Trap37 antibody, dCRSP34 antibody, dCrsp34 antibody, dTRAP37 antibody, med27 antibody, crsp8 antibody, zgc:66302 antibody, MED27 antibody, DDBDRAFT_0216764 antibody, DDBDRAFT_0266847 antibody, DDB_0216764 antibody, DDB_0266847 antibody, 1500015J03Rik antibody, 2310042P07Rik antibody, AA682045 antibody, Crsp8 antibody, D2Ertd434e antibody, RGD1564993 antibody, DKFZp459I1912 antibody, CRSP complex subunit 8-B antibody, Xcrsp34 antibody, crap34 antibody, crsp34 antibody, crsp8-B antibody, med27-a antibody, med27-b antibody, trap37 antibody, mediator complex subunit 27 antibody, Mediator complex subunit 27 antibody, cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa antibody, hypothetical protein antibody, mediator complex subunit 27 S homeolog antibody, MED27 antibody, med27 antibody, crsp8 antibody, Med27 antibody, med27.S antibody
- Background
- He activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. CRSP8 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. CRSP8 is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
- Molecular Weight
- 35 kDa
- Gene ID
- 9442
- NCBI Accession
- NP_004260
- UniProt
- Q6P2C8
- Pathways
- Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
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