AFT1 antibody

Catalog No. ABIN2461742

The Rabbit Polyclonal anti-AFT1 antibody has been validated for WB and ELISA. It is suitable to detect AFT1 in samples from Human, Mouse, Rat and Dog.

Quick Overview for AFT1 antibody (ABIN2461742)

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-AFT1 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATF1.

Application Details

Application Notes: ATF1 antibody can be used for detection of ATF1 by ELISA at 1:312500. ATF1 antibody can be used for detection of ATF1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ATF1 antibody at -20 °C.

Target Details for AFT1

Target: AFT1 (Activating Transcription Factor 1 (AFT1))

Alternative Name: ATF1

Background: ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT [AC] [AG]-3'), a sequence present in many viral and cellular promoters. ATF1 binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes.

Molecular Weight: 29 kDa

Gene ID: 466

NCBI Accession: NP_005162

UniProt: P18846

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades