ELK1 antibody

Catalog No. ABIN2461748

The Rabbit Polyclonal anti-ELK1 antibody has been validated for WB and ELISA. It is suitable to detect ELK1 in samples from Human, Mouse, Rat and Dog.

Quick Overview for ELK1 antibody (ABIN2461748)

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ELK1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ELK1.

Application Details

Application Notes: ELK1 antibody can be used for detection of ELK1 by ELISA at 1:312500. ELK1 antibody can be used for detection of ELK1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ELK1 antibody at -20 °C.

Target Details for ELK1

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Alternative Name: ELK1

Background: ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. ELK1 is a nuclear target for the ras-raf-MAPK signaling cascade.This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade.

Molecular Weight: 45 kDa

Gene ID: 2002

NCBI Accession: NP_005220

UniProt: P19419

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling