KAP1 antibody

Catalog No. ABIN2461766

The Rabbit Polyclonal anti-KAP1 antibody has been validated for WB and ELISA. It is suitable to detect KAP1 in samples from Human and Dog.

Quick Overview for KAP1 antibody (ABIN2461766)

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-KAP1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TRIM28.

Application Details

Application Notes: TRIM28 antibody can be used for detection of TRIM28 by ELISA at 1:312500. TRIM28 antibody can be used for detection of TRIM28 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store TRIM28 antibody at -20 °C.

Target Details for KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: TRIM28

Background: TRIM28 mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. TRIM28 localizes to the nucleus and is thought to associate with specific chromatin regions. It is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING,a B-box type 1 and a B-box type 2, and a coiled-coil region.

Molecular Weight: 89 kDa

Gene ID: 10155

NCBI Accession: NP_005753

UniProt: Q13263

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus