PDLIM5 antibody

Catalog No. ABIN2461790

The Rabbit Polyclonal anti-PDLIM5 antibody is suitable to detect PDLIM5 in samples from Human, Mouse, Rat, Dog and Zebrafish (Danio rerio). It has been validated for WB and ELISA.

Quick Overview for PDLIM5 antibody (ABIN2461790)

Target: PDLIM5 (PDZ and LIM Domain 5 (PDLIM5))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDLIM5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PDLIM5 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDLIM5.

Application Details

Application Notes: PDLIM5 antibody can be used for detection of PDLIM5 by ELISA at 1:1562500. PDLIM5 antibody can be used for detection of PDLIM5 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PDLIM5 antibody at -20 °C.

Target Details for PDLIM5

Target: PDLIM5 (PDZ and LIM Domain 5 (PDLIM5))

Alternative Name: PDLIM5

Background: PDLIM5 is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized.The protein encoded by this gene is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized.

Molecular Weight: 64 kDa

Gene ID: 10611

NCBI Accession: NP_006448

UniProt: Q96HC4