Interleukin enhancer-binding factor 3 (ILF3) antibody

Catalog No. ABIN2461830

This anti- antibody is a Rabbit Polyclonal antibody detecting in WB and ELISA. Suitable for Human and Dog.

Quick Overview for Interleukin enhancer-binding factor 3 (ILF3) antibody (ABIN2461830)

Target: Interleukin enhancer-binding factor 3 (ILF3)

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Product Details

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ILF3.

Application Details

Application Notes: ILF3 antibody can be used for detection of ILF3 by ELISA at 1:62500. ILF3 antibody can be used for detection of ILF3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ILF3 antibody at -20 °C.

Target Details

Target: Interleukin enhancer-binding factor 3 (ILF3)

Alternative Name: ILF3

Background: ILF3 may facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. ILF3 can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 can regulate protein arginine N-methyltransferase 1 activity. ILF3 may regulate transcription of the IL2 gene during T-cell activation. It can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.

Molecular Weight: 95 kDa

Gene ID: 3609

NCBI Accession: NP_036350

UniProt: Q12906

Pathways: M Phase