NFYC antibody

Catalog No. ABIN2461844

The Rabbit Polyclonal anti-NFYC antibody has been validated for WB, IHC and ELISA. It is suitable to detect NFYC in samples from Human, Mouse, Rat and Dog.

Quick Overview for NFYC antibody (ABIN2461844)

Target: NFYC (Nuclear Transcription Factor Y, gamma (NFYC))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFYC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-NFYC Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFYC.

Application Details

Application Notes: NFYC antibody can be used for detection of NFYC by ELISA at 1:62500. NFYC antibody can be used for detection of NFYC by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NFYC antibody at -20 °C.

Target Details for NFYC

Target: NFYC (Nuclear Transcription Factor Y, gamma (NFYC))

Alternative Name: NFYC

Background: NFYC is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. NFYC forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity.The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence, protein sequence alignments and experiments with mutants. Additional regulation, preliminarily supported by the EST database, may be represented by alternative splicing in this subunit.

Molecular Weight: 37 kDa

Gene ID: 4802

NCBI Accession: NP_055038

UniProt: Q13952

Pathways: Regulation of Lipid Metabolism by PPARalpha