RPL32 antibody

Catalog No. ABIN2462057

The Rabbit Polyclonal anti-RPL32 antibody has been validated for WB and ELISA. It is suitable to detect RPL32 in samples from Human, Mouse, Rat and Dog.

Quick Overview for RPL32 antibody (ABIN2462057)

Target: RPL32 (Ribosomal Protein L32 (RPL32))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPL32 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RPL32 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RPL32.

Application Details

Application Notes: RPL32 antibody can be used for detection of RPL32 by ELISA at 1:312500. RPL32 antibody can be used for detection of RPL32 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RPL32 antibody at -20 °C.

Target Details for RPL32

Target: RPL32 (Ribosomal Protein L32 (RPL32))

Alternative Name: RPL32

Background: RPL32 is a ribosomal protein that is a component of the 60S subunit. RPL32 belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene.

Molecular Weight: 15 kDa, 16 kDa, 16 kDa, 16 kDa, 16 kDa, 16 kDa, 16 kDa, 16 kDa

Gene ID: 6161

NCBI Accession: NP_000985

UniProt: P62910