RPS14 antibody

Catalog No. ABIN2462095

This Rabbit Polyclonal antibody specifically detects RPS14 in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat, Dog, Zebrafish (Danio rerio) and Drosophila melanogaster.

Quick Overview for RPS14 antibody (ABIN2462095)

Target: RPS14 (Ribosomal Protein S14 (RPS14))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RPS14 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RPS14.

Application Details

Application Notes: RPS14 antibody can be used for detection of RPS14 by ELISA at 1:12500. RPS14 antibody can be used for detection of RPS14 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RPS14 antibody at -20 °C.

Target Details for RPS14

Target: RPS14 (Ribosomal Protein S14 (RPS14))

Alternative Name: RPS14

Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS14 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 17 kDa, 16 kDa, 16 kDa

Gene ID: 6208

NCBI Accession: NP_001020242

UniProt: P62263

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly