RPS14 antibody (Middle Region)

Catalog No. ABIN2778744

The Rabbit Polyclonal anti-RPS14 antibody is suitable to detect RPS14 in samples from Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for RPS14 antibody (Middle Region) (ABIN2778744)

Target: RPS14 (Ribosomal Protein S14 (RPS14))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS14 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RPS14 Antibody

Binding Specificity: Middle Region

Purpose: RPS14 Antibody - middle region

Sequence: GNRTKTPGPG AQSALRALAR SGMKIGRIED VTPIPSDSTR RKGGRRGRRL

Predicted Reactivity: Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against RPS14. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RPS14

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8110691

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RPS14

Target: RPS14 (Ribosomal Protein S14 (RPS14))

Alternative Name: RPS14

Background:

Background Information: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS14 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor.Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Gene Name: Ribosomal protein S14

Alternative Symbols: S14, EMTB

Protein Name: 40S ribosomal protein S14

Molecular Weight: 17kDa

Gene ID: 6208

NCBI Accession: NP_001020242

UniProt: P62263

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly