EIF4A2 antibody

Catalog No. ABIN2462113

This Rabbit Polyclonal antibody specifically detects EIF4A2 in WB and ELISA. It exhibits reactivity toward Human, Rat, Mouse and Dog.

Quick Overview for EIF4A2 antibody (ABIN2462113)

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Reactivity: Human, Rat, Mouse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4A2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EIF4A2 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF4A2.

Application Details

Application Notes: EIF4A2 antibody can be used for detection of EIF4A2 by ELISA at 1:312500. EIF4A2 antibody can be used for detection of EIF4A2 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store EIF4A2 antibody at -20 °C.

Target Details for EIF4A2

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Alternative Name: EIF4A2

Background: EIF4A2 contains 1 helicase C-terminal domain and 1 helicase ATP-binding domain. It belongs to the DEAD box helicase family. eIF4A subfamily. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5' untranslated region of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Molecular Weight: 45 kDa

Gene ID: 1974

NCBI Accession: NP_001958

UniProt: Q14240