KARS antibody

Catalog No. ABIN2462190

The Rabbit Polyclonal anti-KARS antibody (ABIN2462190) specifically detects KARS in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for KARS antibody (ABIN2462190)

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KARS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-KARS Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KARS.

Application Details

Application Notes: KARS antibody can be used for detection of KARS by ELISA at 1:312500. KARS antibody can be used for detection of KARS by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store KARS antibody at -20 °C.

Target Details for KARS

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Alternative Name: KARS

Background: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

Molecular Weight: 66 kDa

Gene ID: 3735

NCBI Accession: NP_005539

UniProt: Q15046