CPSF4 antibody

Catalog No. ABIN2462224

Product Details anti-CPSF4 Antibody

Target: CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPSF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CPSF4.

Application Details

Application Notes: CPSF4 antibody can be used for detection of CPSF4 by ELISA at 1:62500. CPSF4 antibody can be used for detection of CPSF4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CPSF4 antibody at -20 °C.

Target Details for CPSF4

Target: CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))

Alternative Name: CPSF4 (CPSF4 Products)

Background: Inhibition of the nuclear export of poly (A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs.

Molecular Weight: 24 kDa, 28 kDa, 30 kDa

Gene ID: 10898

UniProt: O95639