CDKN2AIP antibody

Catalog No. ABIN2462272

This Rabbit Polyclonal antibody specifically detects CDKN2AIP in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for CDKN2AIP antibody (ABIN2462272)

Target: CDKN2AIP (CDKN2A Interacting Protein (CDKN2AIP))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDKN2AIP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-CDKN2AIP Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CARF.

Application Details

Application Notes: CARF antibody can be used for detection of CARF by ELISA at 1:312500. CARF antibody can be used for detection of CARF by western blot at 0.3125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CARF antibody at -20 °C.

Target Details for CDKN2AIP

Target: CDKN2AIP (CDKN2A Interacting Protein (CDKN2AIP))

Alternative Name: CARF

Background: CARF was first cloned as a novel binding partner of ARF from a yeast-interactive screen. CARF and ARF colocalize in the perinucleolar region and have a collaborative function. In the nucleoplasm, CARF interacts with p53 and enhances its function. The p53 downregulates CARF in a negative feedback regulatory loop and may also involve p53 antagonist HDM2.

Molecular Weight: 64 kDa

Gene ID: 55602

NCBI Accession: NP_060102

UniProt: Q9NXV6