ALAD antibody

Catalog No. ABIN2462445

This anti-ALAD antibody is a Rabbit Polyclonal antibody detecting ALAD in WB and ELISA. Suitable for Human and Dog.

Quick Overview for ALAD antibody (ABIN2462445)

Target: ALAD (Aminolevulinate Dehydratase (ALAD))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALAD antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ALAD Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALAD.

Application Details

Application Notes: ALAD antibody can be used for detection of ALAD by ELISA at 1:312500. ALAD antibody can be used for detection of ALAD by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ALAD antibody at -20 °C.

Target Details for ALAD

Target: ALAD (Aminolevulinate Dehydratase (ALAD))

Alternative Name: ALAD

Background: The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 Molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria.The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 Molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway, zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternatively spliced transcript variants encoding different isoforms have been identified.

Molecular Weight: 39 kDa, 37 kDa

Gene ID: 210

NCBI Accession: NP_001003945

UniProt: Q6ZMU0