PKLR antibody

Catalog No. ABIN2462457

This anti-PKLR antibody is a Rabbit Polyclonal antibody detecting PKLR in WB, ELISA and IHC. Suitable for Human, Mouse, Rat, Dog and C. elegans.

Quick Overview for PKLR antibody (ABIN2462457)

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Reactivity: Human, Mouse, Rat, Dog, C. elegans

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKLR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-PKLR Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PKLR.

Application Details

Application Notes: PKLR antibody can be used for detection of PKLR by ELISA at 1:62500. PKLR antibody can be used for detection of PKLR by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PKLR antibody at -20 °C.

Target Details for PKLR

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Alternative Name: PKLR

Background: PKLR is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Alternatively spliced transcript variants encoding distinct isoforms have been described.

Molecular Weight: 58 kDa, 62 kDa

Gene ID: 5313

NCBI Accession: NP_870986

Pathways: Ribonucleoside Biosynthetic Process