NAMPT antibody

Catalog No. ABIN2462556

This anti-NAMPT antibody is a Rabbit Polyclonal antibody detecting NAMPT in WB and ELISA. Suitable for Human, Rat, Mouse, Zebrafish (Danio rerio) and Dog.

Quick Overview for NAMPT antibody (ABIN2462556)

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAMPT antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NAMPT Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PBEF1.

Application Details

Application Notes: PBEF1 antibody can be used for detection of PBEF1 by ELISA at 1:312500. PBEF1 antibody can be used for detection of PBEF1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PBEF1 antibody at -20 °C.

Target Details for NAMPT

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: PBEF1

Background: PBEF1 catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

Molecular Weight: 54 kDa

Gene ID: 10135

NCBI Accession: NP_005737

UniProt: P43490