FADS1 antibody

Catalog No. ABIN2462576

The Rabbit Polyclonal anti-FADS1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect FADS1 in samples from Human, Mouse, Rat and Dog.

Quick Overview for FADS1 antibody (ABIN2462576)

Target: FADS1 (Fatty Acid Desaturase 1 (FADS1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FADS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-FADS1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FADS1.

Application Details

Application Notes: FADS1 antibody can be used for detection of FADS1 by ELISA at 1:312500. FADS1 antibody can be used for detection of FADS1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store FADS1 antibody at -20 °C.

Target Details for FADS1

Target: FADS1 (Fatty Acid Desaturase 1 (FADS1))

Alternative Name: FADS1

Background: FADS1 is a member of the fatty acid desaturase (FADS) family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs.The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1, this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.

Molecular Weight: 49 kDa

Gene ID: 3992

NCBI Accession: NP_037534

UniProt: O60427

Pathways: Regulation of Lipid Metabolism by PPARalpha