S1PR5 antibody

Catalog No. ABIN2462615

The Rabbit Polyclonal anti-S1PR5 antibody has been validated for WB, ELISA and IHC. It is suitable to detect S1PR5 in samples from Human, Mouse, Rat, Dog and Drosophila melanogaster.

Quick Overview for S1PR5 antibody (ABIN2462615)

Target: S1PR5 (Sphingosine-1-Phosphate Receptor 5 (S1PR5))

Reactivity: Human, Mouse, Rat, Dog, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-S1PR5 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EDG8.

Application Details

Application Notes: EDG8 antibody can be used for detection of EDG8 by ELISA at 1:312500. EDG8 antibody can be used for detection of EDG8 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store EDG8 antibody at -20 °C.

Target Details for S1PR5

Target: S1PR5 (Sphingosine-1-Phosphate Receptor 5 (S1PR5))

Alternative Name: EDG8

Background: EDG8 is a receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. It Is coupled to both the G (i/0)alpha and G (12) subclass of heteromeric G-proteins (By similarity). It may play a regulatory role in the transformation of radial glial cells into astrocytes and may affect proliferative activity of these cells.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

Molecular Weight: 16 kDa, 17 kDa, 14 kDa

Gene ID: 53637

UniProt: Q9H228