Occludin antibody

Catalog No. ABIN2462645

The Rabbit Polyclonal anti-Occludin antibody has been validated for WB and ELISA. It is suitable to detect Occludin in samples from Human, Mouse, Rat and Dog.

Quick Overview for Occludin antibody (ABIN2462645)

Target: Occludin (OCLN)

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Occludin Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human OCLN.

Application Details

Application Notes: OCLN antibody can be used for detection of OCLN by ELISA at 1:1562500. OCLN antibody can be used for detection of OCLN by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store OCLN antibody at -20 °C.

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: OCLN

Background: OCLN is an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described.This gene encodes an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction. The possibility of several alternatively spliced products has been suggested but the full nature of these products has not been described. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molecular Weight: 59 kDa

Gene ID: 4950

NCBI Accession: NP_002529

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C