FBXO25 antibody

Catalog No. ABIN2462666

The Rabbit Polyclonal anti-FBXO25 antibody has been validated for WB, ELISA and IHC. It is suitable to detect FBXO25 in samples from Human, Dog and Zebrafish (Danio rerio).

Quick Overview for FBXO25 antibody (ABIN2462666)

Target: FBXO25 (F-Box Protein 25 (FBXO25))

Reactivity: Human, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-FBXO25 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FBXO25.

Application Details

Application Notes: FBXO25 antibody can be used for detection of FBXO25 by ELISA at 1:62500. FBXO25 antibody can be used for detection of FBXO25 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store FBXO25 antibody at -20 °C.

Target Details for FBXO25

Target: FBXO25 (F-Box Protein 25 (FBXO25))

Alternative Name: FBXO25

Background: FBXO25 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXO25 belongs to the Fbxs class.

Molecular Weight: 16 kDa, 34 kDa, 42 kDa, 43 kDa

Gene ID: 26260

UniProt: Q8TCJ0