FBXO25 antibody (N-Term)

Catalog No. ABIN2774695

The Rabbit Polyclonal anti-FBXO25 antibody (ABIN2774695) specifically detects FBXO25 in WB. The antibody is reactive with Human, Mouse, Dog, Zebrafish (Danio rerio), Horse, Rat, Guinea Pig, Cow, Rabbit and Pig samples.

Quick Overview for FBXO25 antibody (N-Term) (ABIN2774695)

Target: FBXO25 (F-Box Protein 25 (FBXO25))

Reactivity: Human, Mouse, Dog, Zebrafish (Danio rerio), Horse, Rat, Guinea Pig, Cow, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO25 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FBXO25 Antibody

Binding Specificity: N-Term

Purpose: FBXO25 Antibody - N-terminal region

Sequence: LGEAFNRLDF SSAIQDIRRF NYVVKLLQLI AKSQLTSLSG VAQKNYFNIL

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 93%, Rat: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against FBXO25. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FBXO25

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43117-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for FBXO25

Target: FBXO25 (F-Box Protein 25 (FBXO25))

Alternative Name: FBXO25

Background:

Background Information: FBXO25 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Gene Name: F-box protein 25

Alternative Symbols: FBX25

Protein Name: F-box only protein 25

Molecular Weight: 34kDa

Gene ID: 26260

NCBI Accession: NP_036305

UniProt: Q8TCJ0