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UBE2J1 antibody

This anti-UBE2J1 antibody is a Rabbit Polyclonal antibody detecting UBE2J1 in WB, IHC and ELISA. Suitable for Human and Mouse.
Catalog No. ABIN2462673

Quick Overview for UBE2J1 antibody (ABIN2462673)

Target

See all UBE2J1 Antibodies
UBE2J1 (Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))

Reactivity

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  • 1
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Human, Mouse

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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This UBE2J1 antibody is un-conjugated

Application

  • 33
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  • 6
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Western Blotting (WB), Immunohistochemistry (IHC), ELISA
  • Purification

    Antibody is purified by protein A chromatography method.

    Immunogen

    Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2J1.
  • Application Notes

    UBE2J1 antibody can be used for detection of UBE2J1 by ELISA at 1:1562500. UBE2J1 antibody can be used for detection of UBE2J1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

    Concentration

    1 mg/mL

    Buffer

    Antibody is lyophilized in PBS buffer with 2 % sucrose.

    Handling Advice

    As with any antibody avoid repeat freeze-thaw cycles.

    Storage

    4 °C/-20 °C

    Storage Comment

    For short periods of storage (days) store at 4 °C. For longer periods of storage, store UBE2J1 antibody at -20 °C.
  • Target

    UBE2J1 (Ubiquitin-Conjugating Enzyme E2, J1, U (UBE2J1))

    Alternative Name

    UBE2J1

    Background

    The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2J1 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.

    Molecular Weight

    35 kDa

    Gene ID

    51465

    NCBI Accession

    NP_057105

    UniProt

    Q9Y385
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