PFKL antibody

Catalog No. ABIN2462900

The Rabbit Polyclonal anti-PFKL antibody has been validated for WB and ELISA. It is suitable to detect PFKL in samples from Human, Mouse, Rat and Dog.

Quick Overview for PFKL antibody (ABIN2462900)

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKL antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PFKL Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PFKL.

Application Details

Application Notes: PFKL antibody can be used for detection of PFKL by ELISA at 1:312500. PFKL antibody can be used for detection of PFKL by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PFKL antibody at -20 °C.

Target Details for PFKL

Target: PFKL (Phosphofructokinase, Liver (PFKL))

Alternative Name: PFKL

Background: Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. PFK from muscle is a homotetramer of M subunit, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. PFKL represents the L subunit.

Molecular Weight: 64 kDa, 90 kDa, 85 kDa

Gene ID: 5211

UniProt: Q7L2M7

Pathways: Negative Regulation of Hormone Secretion, Warburg Effect