RRM1 antibody

Catalog No. ABIN2462920

This Rabbit Polyclonal antibody specifically detects RRM1 in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat, Zebrafish (Danio rerio), Dog and C. elegans.

Quick Overview for RRM1 antibody (ABIN2462920)

Target: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Dog, C. elegans

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RRM1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RRM1.

Application Details

Application Notes: RRM1 antibody can be used for detection of RRM1 by ELISA at 1:62500. RRM1 antibody can be used for detection of RRM1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RRM1 antibody at -20 °C.

Target Details for RRM1

Target: RRM1 (Ribonucleotide Reductase M1 (RRM1))

Alternative Name: RRM1

Background: RRM1 is one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. Its gene is located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. Its gene may play a role in malignancies and disease that involve this region.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes one of two non-identical subunits which constitute ribonucleoside-diphosphate reductase, an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region. This gene is oriented in a head-to-tail configuration with the stromal interaction molecule 1 gene (STIM1), with the 3' end of STIM1 situated 1.6 kb from the 5' end of this gene.

Molecular Weight: 90 kDa

Gene ID: 6240

NCBI Accession: NP_001024

UniProt: P23921

Pathways: Positive Regulation of Endopeptidase Activity