RFC3 antibody

Catalog No. ABIN2462934

This anti-RFC3 antibody is a Rabbit Polyclonal antibody detecting RFC3 in WB and ELISA. Suitable for Human and Dog.

Quick Overview for RFC3 antibody (ABIN2462934)

Target: RFC3 (Replication Factor C (Activator 1) 3, 38kDa (RFC3))

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RFC3 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RFC3.

Application Details

Application Notes: RFC3 antibody can be used for detection of RFC3 by ELISA at 1:62500. RFC3 antibody can be used for detection of RFC3 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RFC3 antibody at -20 °C.

Target Details for RFC3

Target: RFC3 (Replication Factor C (Activator 1) 3, 38kDa (RFC3))

Alternative Name: RFC3

Background: The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. RFC3 is the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits.The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 38 kD subunit. This subunit is essential for the interaction between the 140 kD subunit and the core complex that consists of the 36, 37, and 40 kD subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Molecular Weight: 40 kDa

Gene ID: 5983

NCBI Accession: NP_002906

UniProt: P40938

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA