FEN1 antibody

Catalog No. ABIN2462939

The Rabbit Polyclonal anti-FEN1 antibody has been validated for WB and ELISA. It is suitable to detect FEN1 in samples from Human, Mouse, Rat and Dog.

Quick Overview for FEN1 antibody (ABIN2462939)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FEN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FEN1 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human FEN1.

Application Details

Application Notes: FEN1 antibody can be used for detection of FEN1 by ELISA at 1:62500. FEN1 antibody can be used for detection of FEN1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store FEN1 antibody at -20 °C.

Target Details for FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Alternative Name: FEN1

Background: FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Molecular Weight: 42 kDa

Gene ID: 2237

NCBI Accession: NP_004102

UniProt: P39748

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA