DDOST antibody

Catalog No. ABIN2462987

The Rabbit Polyclonal anti-DDOST antibody is suitable to detect DDOST in samples from Human, Mouse, Rat, Dog and Zebrafish (Danio rerio). It has been validated for WB and ELISA.

Quick Overview for DDOST antibody (ABIN2462987)

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDOST antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DDOST Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDOST.

Application Details

Application Notes: DDOST antibody can be used for detection of DDOST by ELISA at 1:62500. DDOST antibody can be used for detection of DDOST by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store DDOST antibody at -20 °C.

Target Details for DDOST

Target: DDOST (Dolichyl-diphosphooligosaccharide--Protein Glycosyltransferase (DDOST))

Alternative Name: DDOST

Background: DDOST is a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. DDOST is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.

Molecular Weight: 46 kDa

Gene ID: 1650

NCBI Accession: NP_005207

UniProt: Q5VWA5

Pathways: Cell RedoxHomeostasis