NAGS antibody

Catalog No. ABIN2463410

The Rabbit Polyclonal anti-NAGS antibody is suitable to detect NAGS in samples from Human, Mouse, Rat and Dog. It has been validated for WB and ELISA.

Quick Overview for NAGS antibody (ABIN2463410)

Target: NAGS (N-Acetylglutamate Synthase (NAGS))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAGS antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NAGS Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NAGS.

Application Details

Application Notes: NAGS antibody can be used for detection of NAGS by ELISA at 1:312500. NAGS antibody can be used for detection of NAGS by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NAGS antibody at -20 °C.

Target Details for NAGS

Target: NAGS (N-Acetylglutamate Synthase (NAGS))

Alternative Name: NAGS

Background: NAGS is a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAG is a cofactor of carbamyl phosphate synthetase I (CPSI), the first enzyme of the urea cycle in mammals. Deficiencies in N-acetylglutamate synthase have been associated with hyperammonemia.The N-acetylglutamate synthase gene encodes a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAG is a cofactor of carbamyl phosphate synthetase I (CPSI), the first enzyme of the urea cycle in mammals. This gene may regulate ureagenesis by altering NAG availability and, thereby, CPSI activity. Deficiencies in N-acetylglutamate synthase have been associated with hyperammonemia.

Molecular Weight: 58 kDa

Gene ID: 162417

NCBI Accession: NP_694551

UniProt: Q8N159