MAPK14 antibody

Catalog No. ABIN2463688

This Rabbit Polyclonal antibody specifically detects MAPK14 in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for MAPK14 antibody (ABIN2463688)

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-MAPK14 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MAPK14.

Application Details

Application Notes: MAPK14 antibody can be used for detection of MAPK14 by ELISA at 1:62500. MAPK14 antibody can be used for detection of MAPK14 by western blot at 0.06 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MAPK14 antibody at -20 °C.

Target Details for MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Alternative Name: MAPK14

Background: MAPK14 encodes a protein which is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.

Molecular Weight: 41 kDa, 41 kDa

Gene ID: 1432

NCBI Accession: NP_001306

UniProt: Q16539

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins