The Rabbit Polyclonal anti-CHRNA3 antibody (ABIN2463729) specifically detects CHRNA3 in WB and ELISA.
The antibody is reactive with Human and Dog samples.
CHRNA3 antibody can be used for detection of CHRNA3 by ELISA at 1:312500. CHRNA3 antibody can be used for detection of CHRNA3 by western blot at 0.1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store CHRNA3 antibody at -20 °C.
The CHRNA3 subunit is expressed in the soma of the majority of pyramidal cells, with the most alpha 3 immunoreactivity observed in CA2-4 and entorhinal cortex and relatively less in CA1 and subicular pyramidal cell soma.