The Rabbit Polyclonal anti-SMAD3 antibody has been validated for WB, ELISA and IHC. It is suitable to detect SMAD3 in samples from Human, Mouse, Rat, Zebrafish (Danio rerio) and Dog.
KO Validated
SMAD3
Reactivity: Human
WB, IHC, IF, IP
Host: Rabbit
Monoclonal
unconjugated
Application Notes
SMAD3 antibody can be used for detection of SMAD3 by ELISA at 1:62500. SMAD3 antibody can be used for detection of SMAD3 by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store SMAD3 antibody at -20 °C.
Target
SMAD3
(SMAD, Mothers Against DPP Homolog 3 (SMAD3))
Alternative Name
SMAD3
Background
SMAD3 is a member of Smad family proteins. SMAD family is known to be important cytoplasmic mediators of signals from the transforming growth factor-beta (TGF-beta) receptor serine/threonine kinases.