TCF4 antibody

Catalog No. ABIN2463811

This Rabbit Polyclonal antibody specifically detects TCF4 in WB and ELISA. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for TCF4 antibody (ABIN2463811)

Target: TCF4 (Transcription Factor 4 (TCF4))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TCF4 Antibody

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TCF4.

Application Details

Application Notes: TCF4 antibody can be used for detection of TCF4 by ELISA at 1:62500. TCF4 antibody can be used for detection of TCF4 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store TCF4 antibody at -20 °C.

Target Details for TCF4

Target: TCF4 (Transcription Factor 4 (TCF4))

Alternative Name: TCF4

Background: TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well.TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well. TCF4 is known to produce multiple transcripts, however as the complete structure is only known for the transcript that encodes the b isoform, that is the variant presented here.

Molecular Weight: 71 kDa

Gene ID: 6925

NCBI Accession: NP_003190

UniProt: P15884

Pathways: WNT Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus