SRF antibody

Catalog No. ABIN2463837

The Rabbit Polyclonal anti-SRF antibody (ABIN2463837) specifically detects SRF in WB and ELISA. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for SRF antibody (ABIN2463837)

Target: SRF (Serum Response Factor (SRF))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SRF Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SRF.

Application Details

Application Notes: SRF antibody can be used for detection of SRF by ELISA at 1:1562500. SRF antibody can be used for detection of SRF by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store SRF antibody at -20 °C.

Target Details for SRF

Target: SRF (Serum Response Factor (SRF))

Alternative Name: SRF

Background: SRF is a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. This gene is the downstream target of many pathways, for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).

Molecular Weight: 52 kDa

Gene ID: 6722

NCBI Accession: NP_003122

UniProt: P11831