SUB1 antibody

Catalog No. ABIN2463866

The Rabbit Polyclonal anti-SUB1 antibody is suitable to detect SUB1 in samples from Human, Mouse, Rat, Dog and Zebrafish (Danio rerio). It has been validated for WB, ELISA and IHC.

Quick Overview for SUB1 antibody (ABIN2463866)

Target: SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-SUB1 Antibody

Purification: Antibody is supplied as total IgG.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PC4.

Application Details

Application Notes: PC4 antibody can be used for detection of PC4 by ELISA at 1:7812500. PC4 antibody can be used for detection of PC4 by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 200 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PC4 antibody at -20 °C.

Target Details for SUB1

Target: SUB1 (Activated RNA Polymerase II Transcriptional Coactivator p15 (SUB1))

Alternative Name: PC4

Background: PC4 (activated RNA polymerase II transcription cofactor 4) is a transcriptional coactivator, possessing the ability to suppress promoter-driven as well as nonspecific transcription via its DNA binding activity. The repressive activity of PC4 on promoter-driven transcription is alleviated by transcription factor TFIIH. TFIIH protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 through the ERCC3 helicase activity.

Molecular Weight: 14 kDa

Gene ID: 10923

NCBI Accession: NP_006704

UniProt: P53999