ELK1 antibody (truncated)

Catalog No. ABIN2464047

This anti-ELK1 antibody is a Mouse Monoclonal antibody detecting ELK1 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for ELK1 antibody (truncated) (ABIN2464047)

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ELK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 3H6D12-4H9C8-4H9F1

Product Details anti-ELK1 Antibody

Binding Specificity: truncated

Immunogen: Ni-NTA purified truncated recombinant ELK1 expressed in E. Coli strain BL21 (DE3).

Isotype: IgG1

Application Details

Application Notes: Western Blot:1:500 - 1:1,000
IHC(P):1:500 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: ELK1 monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for ELK1

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Alternative Name: ELK1

Background: The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily,which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus,It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.

Gene ID: 2002

UniProt: P19419

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling