FER antibody (truncated)

Catalog No. ABIN2464050

The Mouse Monoclonal anti-FER antibody (Clone 5D2C4-8D8C12) (ABIN2464050) specifically detects FER in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for FER antibody (truncated) (ABIN2464050)

Target: FER (FER Tyrosine Kinase (FER))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FER antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 5D2C4-8D8C12

Product Details anti-FER Antibody

Binding Specificity: truncated

Immunogen: Ni-NTA purified truncated recombinant FER-His expressed in E. Coli strain BL21 (DE3).

Isotype: IgG1

Application Details

Application Notes: Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: FER monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for FER

Target: FER (FER Tyrosine Kinase (FER))

Alternative Name: FER

Background: FER (fer tyrosine kinase) is a member of the FPS/FES family of nontransmembrane receptor tyrosine kinases, which shares a functional domain and is involved in signaling pathways through receptor tyrosine kinases (RTK) and cytokine receptors. The Fes /Fps family is distinct from c-Src, c-Abl and related nRTKs and was originally distinguished as a homolog to retroviral oncoproteins. In vivo, Fer kinase assembles into homotrimers via conserved coiled-coil domains. The N-terminal coiled-coil domains of Fer can autophosphorylate in trans, thereby regulating their cellular function through differential phosphorylation states. Growth factor exposure can induce tyrosine phosphorylation of Fer and recruitment of Fer to RTK complexes containing p85. It is expressed predominantly in mature hematopoietic cells of the granulocytic and monocytic lineage, and has been shown to be expressed in vascular endothelial cells. Fer is implicated in insulin signaling, cell-cell signaling, human prostatic proliferative diseases, and is involved in the regulation of G1 progression.

Gene ID: 2241

UniProt: P16591