MSH2 antibody

Catalog No. ABIN2464085

The Mouse Monoclonal anti-MSH2 antibody is suitable to detect MSH2 in samples from Human. It has been validated for WB, IHC and ELISA.

Quick Overview for MSH2 antibody (ABIN2464085)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MSH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 3A2B8C

Product Details anti-MSH2 Antibody

Immunogen: Ni-NTA purified recombinant human MSH2 expressed in E. Coli strain BL21 (DE3).

Isotype: IgG1

Application Details

Application Notes: Western Blot:Dilution 1:200 - 1:1,000
IHC(P):Dilution 1:200 - 1:1,000
IHC(F):Dilution 1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: MSH2 monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Alternative Name: MSH2

Background: MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50 % of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process.

Gene ID: 4436

UniProt: P43246

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response