MAPKAP Kinase 5 antibody (truncated)

Catalog No. ABIN2464093

This Mouse Monoclonal antibody specifically detects MAPKAP Kinase 5 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for MAPKAP Kinase 5 antibody (truncated) (ABIN2464093)

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAPKAP Kinase 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 7H10B4

Product Details anti-MAPKAP Kinase 5 Antibody

Binding Specificity: truncated

Immunogen: Ni-NTA purified truncated recombinant PRAK-His expressed in E. Coli strain BL21 (DE3).

Isotype: IgG1

Application Details

Application Notes: Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: PRAK monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for MAPKAP Kinase 5

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Alternative Name: PRAK

Background: PRAK (p38-regulated /activated kinase), also referred to as mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK)-5, is an ubiquitously expressed serine/threonine kinase regulated by p38alpha and p38beta MAP kinases. Activated JNK, p38? or p38? are unable to induce phosphorylation of PRAK in vitro. Phosphorylation of PRAK occurs in vivo in response to p38 activation by stress-related extracellular stimuli including UV light, oxidation and proinflammatory cytokines. Two other substrates for p38, MAPKAPK-2 and MAPKAPK-3/3pK, share approximately 45 % sequence homology with PRAK including the phosphorylation motif recognized by p38, Lys-X-Thr-Pro. Activated PRAK has been shown to specifically phosphorylate HSP 27 in vitro, suggesting that the protein may play a role in stress-induced small heat shock protein phosphorylation in vivo.

Gene ID: 8550

UniProt: Q8IW41

Pathways: MAPK Signaling