Actin antibody (N-Term)

Catalog No. ABIN2465349

This anti-Actin antibody is a Goat Polyclonal antibody detecting Actin in WB, IHC, ELISA and IF. Suitable for Human, Rat, Mouse and Pig.

Quick Overview for Actin antibody (N-Term) (ABIN2465349)

Target: Actin

Reactivity: Human, Rat, Mouse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Actin Antibody

Binding Specificity: N-Term

Specificity: VariantsNP_001604.1 and NP_001135417.1 encode the same protein.

Purification: Actin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Actin antibody was raised against a 10 amino acid synthetic peptide near the N-Terminus of Actin.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 45 kDa band observed in human duodenum lysates (calculated MW of 42.0 kDa according to NP_001604.1). Recommended concentration: 0.1-0.3 μg/mL. IHC: In paraffin embedded Human and Murine Lung shows staining of the alveolar walls. Recommended concentration, 2-4 g/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for Actin

Target: Actin

Gene ID: 59

NCBI Accession: NP_001604

UniProt: P62736